胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析

基于传统的实验方法，对胜利油田滩涂区土壤中石油降解菌进行了筛选和鉴定，并利用PCR-DGGE (变性梯度凝胶电泳)技术分析了胜利油田滩涂区的菌群多样性.结果表明：由研究区土壤中筛选出13株石油降解菌，其中，6株石油降解菌的石油降解率>90%，能降解大部分C₁₂～C₂₆的石油烷烃，系统发育学鉴定为Alcanivorax、Halomonas和Marinobacter，均属于γ-proteobacteria分支;胜利油田滩涂区未培养优势菌有Sulfurovum、Gillisia和Arcobacter;该区优势菌群中γ-proteobacteria所占比重较大，其次为α-proteobactiria、ε-proteobactiria、Actinobacteria 和Flavobacteria.

Isolation, identification and diversity analysis of petroleum-degrading bacteria in Shengli Oil Field wetland soil.

The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE (denaturing gradient gel electrophoresis). A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12〖KG-*2〗-〖KG-*7〗C26 petroleum hydrocarbon, with a degradation rate of >90%. These petroleum degraders were phylogeneticly identified as the members of Halomonas, Alcanivorax, and Marinobacter, which were all belonged to γ-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, Gillisia and Arcobacter. Among the predominant bacteria, γ-proteobacteria accounted for a larger proportion, followed by α-proteobactiria, ε-proteobactiria, Actinobacteria, and Flavobacteria.