环境无机汞对泥鳅视网膜S100表达影响

用含低浓度Hg2 的水饲养泥鳅(Misgurnus anguillicaudatus),研究Hg2 污染对视网膜中S100免疫反应阳性结构数量及分布的影响。用免疫组织化学ABC法标记S100免疫阳性(S100-IR)细胞,显微镜下观察S100免疫反应阳性细胞并计数。结果显示,处理组S100首先在神经节细胞层(GCL)、神经纤维层(NFL)表达,然后逐渐在其他各层表达。与对照组相比,处理组S100免疫反应阳性细胞数显著增多,且该变化与环境中无机汞处理时间成正相关;阳性细胞胞体明显膨胀,突起稠密粗大,S100阳性反应强。研究提示,Hg2 的神经毒害作用导致泥鳅视网膜中S100-IR细胞数的增多。推测Hg2 污染可能对视网膜有伤害性影响,而神经胶质细胞在视网膜修复过程中起了重要作用。

Exposure to Inorganic Mercury Affects S100-expression in the Retina of Loaches

Exposing loaches (Misgurnus anguillicaudatus) to water with low concentration of Hg 2 for several weeks,we studied the effect of Hg 2 pollution on the distribution and number of S100-IR structure in the retina.Immunohistochemical ABC method was applied to display the S100 immunoreactive (S100-IR) structure.Under microscope we observed and counted the numbers of S100-IR cells.The S100-IR cells appeared in the nerve fibre layer (NFL),ganglion cell layer (GCL),and then progressively in the other layers.Compared with the control fish,the number of S100-IR cells in poisoned loaches increased significantly and was proportional to exposure time.In addition,the S100 immunoreactive cells showed a swollen cell body with enhanced S100-immunoreactivity.In conclusion,Hg 2 appears to damage retina and glial cells may play an anti-toxic role in retina recovering in loaches.