光照、温度和pH对雨生红球藻光合特性的影响

采用测定光合放氧速率和荧光动力学的方法，研究分析了光照强度、温度和pH对雨生红球藻Haematococcus pluvialis CG-11绿色游动细胞阶段光合作用特性的影响。结果表明，H. pluvialis CG-11光合作用的饱和光强为109.1 μmol/m2·s，最大光合放氧速率为75.9 μmol O2/mg Chla·h；适宜的生长温度范围在25－30℃之间，温度在25℃时光合速率最大；pH在7.5－8.0范围内，光合效率较高，在pH为7.5时放氧速率最大，为75.5 μmol O2/mg Chla·h。在实验pH条件下，H. pluvialis CG-11叶绿素荧光动力学参数呈现出相似的趋势，在6.0－7.5范围内，Fv/ Fm、Fv/ F0、ΦPSⅡ和ETR值随pH升高而增大，pH为7.5时达到最大值，pH超过7.5时，Fv/ Fm和Fv/ F0值明显下降，而ΦPSⅡ和ETR的下降趋势不明显。

Effect of lighting, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11

The effects of light intensity, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11 were studied by measuring photosynthetic O2 evolution and fluorescence kinetics. The results showed that the saturation thetic rate was obtained at 25℃; the photosynthetic efficiency kept quite high at the pH range of 7.5-8.0, with the maxi-range of 7.5-8.0, the Fv/Fm, Fv/Fo, ΦPSⅡ and ETR increased following the rising of pH, with their maximum values at pH 7.5, Fv/Fm and Fv/Fo decreased significantly over pH 7.5, while the decreasing trend didn't change obviously in ΦPSⅡ and ETR.