Localization of tritiated prostaglandin E1 in rat adrenal cortices |
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| Authors: | Dr. David P. Penney J. Olson G. V. Marinetti S. Vaala K. Averill |
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| Affiliation: | (1) Departments of Anatomy and Biochemistry, University of Rochester School of Medicine and Dentistry Rochester, New York, U.S.A. |
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| Abstract: | Summary To determine whether or not prostaglandins enter adrenocortical parenchymal cells,3H-PGE1 was injected intravenously into rats. In histological preparations, grains denoting activity were noted in intracellular lipid droplets and nuclei and in sinusoids. At the fine structural level, activity was observed in lipid droplets, mitochondria, the agranular endoplasmic reticulum, nuclei and the plasma membrane. Biochemical lipid analyses of the adrenals revealed activity in the cholesterol and cholesterol ester fractions. Large amounts of unaltered3H-PGE1 and its degradation products were also present. Compared to the liver, the adrenal was more effective in degrading prostaglandin, when expressed on a weight basis. The possible roles of the organelles in PGE1 degradation and in prostaglandin-related hormone synthesis are discussed. Supported by N.I.H. Grants AM-09561 and RR-05403. |
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| Keywords: | Adrenal cortex Prostaglandin E1 Autoradiography Electron microscopy Lipid analysis |
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