A highly regulated, recording constant power, voltage, and current supply for electrophoresis and isoelectric focusing. |
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Authors: | J M Williams N Catsimpoolas |
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Affiliation: | Department of Microbiology, Biozentrum of the University of Basel, Basel, Switzerland |
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Abstract: | Assays for endonuclease and ligase activities have been devised that make use of the fact that circular phage λ DNA is preferentially retained on nitrocellulose filters while linear λ DNA is not. The assay is rapid, time dependent, and quantitative and has been used for the purification of restriction endonucleases and DNA ligases. |
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