Isolation and characterisation of three fucosyloligosaccharide-1-phosphates from normal human urine |
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Authors: | Gérard Strecker Jean-Michel Wieruszeski Jean-Claude Michalski Jean Montreuil |
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Affiliation: | 1. Laboratoire de Chimie Biologique (Unit Mixte du CNRS no. 111), Université des Sciences et Techniques de Lille Flandres-Artois, 59655, Villeneuve d'Ascq Cedex, France
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Abstract: | Three phosphate-containing fucosyloligosaccharides were isolated from normal human urine using charcoal adsorption, gel-filtration, ion-exchange chromatography and paper chromatography. Chemical investigations and 400 MHz1H-NMR spectroscopy analyses led to the following structures: Their oligosaccharide chains are identical with, or similar to the fucosyloligosaccharides and urinary compounds synthesized by the stepwise transfer ofN-acetylglucosamine, galactose, fucose andN-acetylgalactosamine to free galactose or glucose residues. A transfer reaction of monosaccharides to free hexose-1-phosphates or glycosylnucleotides is proposed for explaining the origin of these sugar-phosphates. |
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