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The kinetics of electron entry in cytochromec oxidase |
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| Authors: | Francesco Malatesta Giovanni Antonin Paolo Sarti Beatrice Vallone Maurizio Brunori |
| Affiliation: | (1) Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universitá di Roma Tor Vergata, Rome, Italy;(2) Dipartimento di Scienze Biomediche, Tecnologia e di Biometria, Universitá dell'Aquila, L'Aquila, Italy;(3) Dipartimento di Scienze Biochimiche, Centro di Biologia Molecolare del CNR, Universitá di Roma La Sapienza, Rome, Italy;(4) Dipartimento di Scienze Biochimiche, Universitá di Roma !["ldquo"]("/content/g1m47x722338l0j1/xlarge8220.gif") La Sapienza!["rdquo"]("/content/g1m47x722338l0j1/xlarge8221.gif") , Piazzale Aldo Moro, 5, I-00185 Rome, Italy |
| Abstract: | Summary The kinetics of electron entry in beef heart cytochromec oxidase have been studied by stopped-flow spectroscopy following chemical modification of the CuA site with mercurials. In this derivative CuA is no longer reducible by cytochrome c while cytochromea may accept electrons from the latter with rates comparable to the native enzyme. The results indicate that CuA is not the exclusive electron entry site in cytochromec oxidase. |
| Keywords: | Electron transfer Chemical modification Cytochrome oxidase |
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