Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens |
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| Authors: | E. M. J. Salentijn N. N. Sandal R. Klein-Lankhorst W. Lange Th. S. M. De Bock K. A. Marcker W. J. Stiekema |
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| Affiliation: | (1) Department of Molecular Biology, DLO — Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O.Box 16, NL-6700 AA Wageningen, The Netherlands;(2) Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark |
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| Abstract: | New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1pat-1locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1pro-1locus. |
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| Keywords: | Molecular markers Beet cyst nematode resistance Hs1 Heterodera schachtii Schm Satellite DNA Monosomic fragment additions Beta Pulsed-field gel electrophoresis |
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