Characterization of a Mutant from Lactobacillus amylovorus JCM 1126T with Improved Utilization of Sucrose |
| |
Authors: | Yuji Oda Miyuki Ito |
| |
Affiliation: | (1) Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan, JP;(2) Department of Applied Biological Science, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan, JP |
| |
Abstract: | A strain YF43, which can grow on sucrose as rapidly as glucose, was isolated by mutation from Lactobacillus amylovorus JCM 1126, the type strain defective in sucrose utilization. Exogenous sucrose stimulated the production of invertase by strains YF43 and JCM 1126 simultaneously. In a medium containing fructooligosaccharide as the sole carbon source, the cells of strain YF43 showed high invertase activity in spite of poor growth. The two invertases produced in the cells grown on sucrose and fructooligosaccharide were an identical β-fructofuranosidase, as judged from properties of partially purified enzymes. These observations indicated that strain YF43 is a mutant improved for permeation of sucrose and not derepressed for the synthesis of invertase. Received: 23 May 2000 / Accepted: 26 June 2000 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|