Kanamycin resistance of germinating pollen of transgenic plants |
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| Authors: | Yu V Chesnokov R Manteuffel |
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| Institution: | (1) Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466 Gatersleben, Germany e-mail: chesnoko@ipk-gatersleben.de Tel.: +49(0)39482 5407, Fax: +49(0)39482 5139, DE;(2) Institute of Genetics, Moldavian Academy of Sciences, Lesnaya Str. 20, MD-2002 Kishinev, Moldova, MD |
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| Abstract: | The influence of kanamycin on the percentage of pollen germination and on tube growth of pollen from non-transformed and transformed
plants of various species containing a chimaeric kanamycin resistance gene (NPTII) was investigated. Pollen grains isolated from kanamycin resistant plants expressed resistance when germinating in vitro, whereas kanamycin impaired tube growth of pollen from non-transformed plants. Pollen grains from transgenic plants were less
sensitive and produced significantly longer tubes. mRNAs of the chimaeric gene are probably presynthesized concurrently with
the other mRNAs during microsporogenesis, and kanamycin resistance is expressed by mRNA translation during pollen tube elongation.
Received: 24 August 1999 / Revision accepted: 20 October 1999 |
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| Keywords: | Kanamycin resistance Pollen Tomato interspecific hybrid Nicotiana plumbaginifolia |
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