Purification and translation of ovalbumin,conalbumin, ovomucoid and lysozyme messenger RNA |
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Authors: | Richard D Palmiter Lynne T Smith |
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Institution: | (1) Dept. of Biochemistry, Searle Research Laboratories, High Wycombe, Bucks., England;(2) Present address: The Biological Laboratories, Harvard University, 02138 Cambridge, Massachusetts, USA |
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Abstract: | Messenger RNAs for 4 egg white proteins (ovalbumin, conalbumin, ovomucoid and lysozyme) were assayed in a cell-free, protein-synthesizing system derived from rabbit reticulocytes. Each of these messengers was purified about 33-fold from hen oviduct polysomal RNA using oligo(dT) cellulose. The apparent minimal translational efficiencies varied from 22 translations for each ovalbumin mRNA to less than 1 for lysozyme mRNA. These messengers are not polycistronic with each other since they have distinct sedimentation values of: lysozyme, 8.5S; ovomucoid, 11S; ovalbumin, 15S; and conalbumin 18S. Several aspects of this system indicate that it will be valuable for dissecting the fine control of mRNA metabolism. |
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