Two classes of continuous cell lines established from Syrian hamster 9 day gestation embryos: Preneoplastic cells and progenitor cells |
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Authors: | Toshimitsu Okeda Yasushi Yokogawa Hiroaki Ueo Mary A Bury Paul O P Ts'o Sarah A Bruce |
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Institution: | (1) Division of Biophysics, School of Hygiene and Public Health, The Johns Hopkins University, 615 N. Wolfe Street, 21205 Baltimore, Maryland;(2) Present address: First Department of Internal Medicine, Oita Medical College, 1-1506 Idaigaoka, Hazamo-Cho, 879-56 Oita, Japan;(3) Present address: Division of Endocrinology and Medicine, Department of Internal Medicine, Hamanomachi General Hospital, 3-5-27 Maizuru, Chuo-ku, 810 Fukuoka, Japan;(4) Present address: Department of Surgery, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, 875 Beppu, Japan |
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Abstract: | Summary Primary cultures of 9-d-gestation Syrian hamster embryo (E9) cells are distinct from primary cultures of later gestational
age in terms of their growth and differentiation. First, primary E9 cell cultures express multiple mesenchymal differentiation
lineages (e.g., adipocyte, myoblast) only rarely seen in cultures of 13-d-gestation fetal (F13) cells. Second, although most
primary E9 cultures have a limited in vitro proliferative life span and exhibit cellular senescence similar to primary cultures
of F13 cells, E9 cultures seem to have higher frequency of escape from senescence and conversion to continuous cell lines
compared to F13 cells. Moreover, this frequency can be further increased 4- to 5-fold by continuous exposure of the E9 cells
to tumor promoters or epidermal growth factor. Eleven continuous cell lines have been isolated from unreated, promoter-treated,
or epidermal growth factor-treated primary E9 cultures. Seven of these are neoplastic or preneoplastic. However, the remaining
four do not show any evidence of being in neoplastic progression and three of these continue to express the same differentiated
phenotype observed in ther parental primary cell cultures.
These studies were supported in part by grants from the National Institutes of Health (AG 01998), Bethesda, MD, and the U.S.
Department of Energy (DE-A-C02-76-EVO-3280), Washington, DC. |
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Keywords: | embryonic cells adipocytes myoblasts tumor promoters epidermal growth factor cellular senescence |
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