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Use of the SSLP-based method for detection of rare apomictic events in a sexual AtSERK1 transgenic Arabidopsis population
Authors:Laksana Kantama  Yolanda Lambert  Haifen Hu  Hans de Jong  Sacco C de Vries  Eugenia Russinova
Institution:(1) Laboratory of Biochemistry, Department of Agrotechnology and Food Sciences, Wageningen University & Research Centre, Dreijenlaan 3, 6703, HA, Wageningen, The Netherlands;(2) Laboratory of Genetics, Wageningen University & Research Centre, Arboretumlaan 4, 6703, BD, Wageningen, The Netherlands;(3) Present address: Faculty of Liberal Arts & Science, Kasetsart University, Kampaengsaen Campus, Malaiman Rd., 73140 Nakorn Pathon, Thailand
Abstract:Here we present a screening method to evaluate the potential of genes to transfer aspects of apomixis into sexual crop plants. Based on the assumption that an apomictic progeny is an exact genetic replica of the mother plant we employed a set of single sequence length polymorphism (SSLP) markers to identify individuals displaying heterozygosity fixation in segregating sexual populations as an indication of rare apomictic events. Here we present the results of such a study using the Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 (AtSERK1) gene expressed under the control of the AtLTP1 promoter in sexual Arabidopsis plants. In one of the three tested F2 transgenic populations expressing the AtLTP1::AtSERK1 construct we observed two plants with heterozygosity maintenance for the full set of SSLP markers indicating a possible clonal inheritance. However, as their offspring revealed a close to binomial segregation for a number of heterozygous loci, it was concluded that these two putative apomictic plants either lost their clonal ability in the next generation or resulted from incidental recombination events displaying the genotype of the parent. Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users.
Keywords:Apomixis            Arabidopsis            SSLP  Adventitious embryony            AtSERK1
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