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Regeneration and transformation of Ribes
Authors:Julie Graham  Ronnie J McNicol
Institution:(1) Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK
Abstract:Transformation of the black currant cv. Ben More was achieved by utilising the binary vector system of Agrobacterium tumefaciens. This system involved the inoculation of peeled internodal stem segments with A. tumefaciens strain LBA4404 containing the binary vector PBI121.X with the marker genes Betaglucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Shoot regeneration occurred on nutrient media based on M&S salts. Transformation was confirmed by the fluorogenic assay procedure which determined that the GUS gene had been transferred into the plant material and was being expressed. Concurrent transfer of the NPTII gene into the plant material was also confirmed with a lsquodot blotrsquo assay on selected GUS positive plantlets.
Keywords:Agrobacterium  regeneration  Ribes nigrum  tissue culture  transformation
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