Lectin-induced alterations on the proliferation of three human prostatic cancer cell lines |
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Authors: | Isabelle Camby Thierry Janssen Robert De Decker Michel Petein Gil Raviv Jean-Lambert Pasteels Robert Kiss André Danguy |
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Institution: | 1. Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium 2. Bruxelles Fonds National de la Recherche Scientifique, Belgium 3. Service d’Urologie, H?pital Erasme, Université Libre de Bruxelles, Bruxelles, Belgium 4. Département de Pathologie, Institut J. Bordet, Bruxelles, Belgium
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Abstract: | Summary While lectins are known to influence the cell growth of several types of normal and neoplastic tissues, their roles in the
case of prostatic cancer cells remain relatively unexplored. In the present work, we report thein vitro influence of five lectins, namely peanut (PNA), wheat germ (WGA), Concanavalin A (Con A),Griffonia simplicifolia (GSA-IA4), andPhaseolus vulgaris (PHA-L) agglutinins, on the cell proliferation of one androgen-sensitive (LNCaP) and two androgen-insensitive (PC-3 and DU
145) human prostatic cancer cell lines cultured in either 10% or 1% fetal bovine serum (FBS)-supplemented media. The cell
proliferation was assessed by means of the colorimetric 3-(4,5-dimethythiazol-2-yle)2,5-diphenyltetrazolium bromide. (MTT)
assay. Four lectin concentrations were tested (i.e., 0.1, 1, 10, and 100 μg/ml) at five experimental states (i.e., 2, 3, 5,
7, and 9 d following the addition of each lectin to the culture media). Our results demonstrated that the five lectins under
study had a globally significant dose-dependent toxic effect on prostatic cancer cell proliferation. Nevertheless, low doses
of GSA-IA4 and PHA-L significantly (P<0.05 toP<0.001) increased the cell proliferation of confluent PC-3 cells. Increasing the FBS from 1% to 10% in the culture media significantly
antagonized lectin-induced toxicity in the three prostatic cell lines. In conclusion, the present data strongly suggest that
some lectins might influence the proliferation of prostatic carcinoma cells. In addition, because lectins are present in our
diet, and are able to pass into the systemic circulation and thus reach the prostate, the present results suggest that some
lectins might exert an influence on prostate cancer growth under clinical conditions. |
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Keywords: | wheat germ agglutinin (WGA) peanut agglutinin (PNA) Griffonia simplicifolia I-A4 agglutinin (GSA-IA4) Phaseolus vulgaris agglutinin (PHA-L) Concanavalin A (Con A) in vitro |
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