Altered transferrin gene expression in preneoplastic and neoplastic liver lesions induced in rats with N-nitrosomorpholine |
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Authors: | K Schiebet H Stumpf H Zerban E Pekel P Bannasch |
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Institution: | 1. Abteilung für Cytopathologie, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, W-6900, Heidelberg, Federal Republic of Germany
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Abstract: | The expression of the gene for the iron transport protein transferrin was found to be altered in preneoplastic and neoplastic
lesions induced in the rat liver by N-nitrosomorpholine. The total RNA of ten hepatocellular carcinomas (HCC) was investigated
by Northern blot analysis using a cDNA-probe comprising 150 bp of the 3′ region and compared with the total hepatic RNA in
untreated rats. Seven hepatocellular carcinomas showed slight or pronounced reduction in transferrin expression. In situ hybridization
of two additional hepatocellular carcinomas revealed marked reduction in the mRNA level for the transferrin gene compared
with the surrounding tissue. In contrast, the majority of early preneoplastic lesions storing excess glycogen and tigroid
cell foci expressed increased levels of transferrin mRNA. The loss of glycogen in mixed cell foci, which represent a later
stage of hepatocarcinogenesis, was usually accompanied by a decrease in transferrin mRNA suggesting a close relationship between
this change in gene expression and cellular dedifferentiation emerging during hepatocarcinogenesis. |
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Keywords: | Transferrin Gene expression Hepatocar-cinogenesis Preneoplastic lesions In situ hybridization |
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