Molecular Species in the Protein Body II (PB-II) of Developing Rice Endosperm |
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| Authors: | Toshio Sugimoto Kunisuke Tanaka Zenzaburo Kasai |
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| Institution: | 1. National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki 305, Japan;2. Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Kyoto 606, Japan;3. Plant Nutrition Laboratory, College of Agricultural Chemistry, Kinki University, Osaka 577, Japan |
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| Abstract: | The molecular weight and subunit composition of glutelin, the major storage protein of rice, in the major type of protein bodies of developing rice seeds was examined by gradient and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Glutelin in the protein body was the assembled from heterogeneous subunits, and the molecular weights were estimated to be 64, 140, 240, 320, 380, and 500 k by gradient SDS-PAGE. High molecular weight proteins (larger than 2,000 k) were also observed.The two-dimensional SDS-PAGE under reduced conditions showed, that the glutelin in the protein body was composed of two groups of polypeptides, 22~23 and 37~39k, bound by disulfide linkages. |
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| Keywords: | segmented filamentous bacteria clostridia IgA breeder |
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