Inhibition Site of Cupric Ions on the Growth of Thiobacillus ferrooxidans on Sulfur-salts Medium |
| |
| Authors: | Tsuyoshi Sugio Kimihito Wada Wataru Mizunashi Kazutami Imai Tatsuo Tano |
| |
| Affiliation: | Department of Agricultural Chemistry, Faculty of Agriculture, Okayama University, Okayama 700, Japan |
| |
| Abstract: | The cDNA sequence coding for tuna growth hormone (tGH) was placed under the control of the repressible acid phosphatase (PHO5) promoter of a yeast, Saccharomyces cerevisiae, in an expression plasmid, pAM82. The yeast cells transformed with the plasmid synthesized tGH only when the cDNA was attached to the vector through a synthetic oligonucleotide linker having a similar sequence to the 5′-flanking region of the PHO5 structural region. The amount of tGH produced in yeast cells accounted for more than 3% of the total cellular protein and the product was immunologically identified as tGH by Western blotting using polyclonal antibodies specific to tGH. |
| |
| Keywords: | |
|
|
