L-Serine Production by Temperature-sensitive Mutants of Methanol-utilizing Bacterium Pseudomonas MS 31 |
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Authors: | Yasushi Morinaga Shigeru Yamanaka Koichi Takinami |
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Affiliation: | Central Research Laboratories of Ajinomoto Co., Kawasaki 210, Japan |
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Abstract: | Temperature-sensitive mutants producing L-serine efficiently from glycine were obtained from the facultative methylotroph Pseudomonas MS 31. Forty-five mutant strains showed adequate growth on methanol at 30°C but little or no growth at 37°C. Fourteen of these mutants produced L- serine more efficiently than the wild-type strain. The typical mutant strain ts 162 showed a high conversion rate in glycine-to-L-serine when the cultivation temperature was changed from a permissive (30°C) to non-permissive state (38?42°C) together with the addition of glycine and methanol after adequate growth. The mutant strain accumulated 6.8 mg L-serine from 12 mg glycine per ml culture under optimum conditions. The reduction of L-serine degrading activity in the mutant strain seemed to contribute to the high productivity of L-serine. |
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Keywords: | carrageenan Salmonella infection host defense macrophage, J774.1 cells |
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