Inhibitory Effects of 2-Deoxy-D-glucose on Methanol Metabolism in Torulopsis sp.

Inhibitory effects of 2-deoxy-D-glucose (2dG) on methanol metabolism in Torulopsis A–12 were investigated. The remarkable inhibition was observed on growth in the presence of 50 μg/ml of 2dG when methanol was used as a carbon source. At the earlier time of incubation with 2dG, the incorporation of ¹⁴C-methanol into hexose-phosphate by intact cells was inhibited by 2dG, and this led to the reduction of the intracellular concentration of hexose-phosphates. In the later period of incubation the specific activity of alcohol oxidase reduced, and formate accumulated extracellularly.