Development of a Host-vector System for Gluconobacter suboxydans |
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| Authors: | Masahiro Fukaya Hajime Okumura Hiroshi Masai Takeshi Uozumi Teruhiko Beppu |
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| Institution: | 1. Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd. Handa-shi, Aichi 475, Japan;2. Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo 113, Japan |
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| Abstract: | A shuttle vector for Gluconobacter suboxydans and Escherichia coli was constructed by ligation of a cryptic plasmid, pMV201, found in G. suboxydans IFO 3130 to E. coli plasmid pACYC177. The chimeric plasmid named pMGlOl carries the ampicillin resistance gene derived from pACYC177 and transforms G. suboxydans var. α IFO 3254 as well as E. coli. The transformation conditions for G. suboxydansvar. α IFO 3254 were examined using pMGlOl DNA. Competent cells were induced efficiently by treatment with LiCl or RbCl CaCl2 which induced the competency of Acetobacter was much less effective. Addition of polyethylene glycol enhanced the transformation efficiency significantly. An efficiency of approximately 102 transformants per μg DNA was finally obtained. |
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