Isolation and Identification of Lutein from Lemna paucicostata 381 as an Inhibitor of Benzoic Acid-induced Flowering in Lemna paucicostata 151 |
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| Authors: | Shozo Fujioka Akira Sakurai Naotsugu Sugimoto Isomaro Yamaguchi Noboru Murofushi Nobutaka Takahashi |
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| Institution: | 1. The Institute of Physical and Chemical Research, Wako, Saitama 351–01, Japan;2. Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku Tokyo 113, Japan |
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| Abstract: | Efforts were made to isolate flower-inhibitory substances from extracts of the short-day plant Lemna paucicostata 381. Lemna paucicostata 151, which was used in the bioassay, exhibits poor flowering in response to the photoperiod, but flowers profusely in response to benzoic acid. Therefore, only those substances that inhibit benzoic acid-induced flowering were studied. Several fractions obtained by silica gel column chromatography exhibited flower-inhibitory activity when tested on L. paucicostata 151. After several purification steps, one of the active principles was identified as lutein by MS, UV and NMR spectroscopic analyses. Lutein and its isomer zeaxanthin inhibited benzoic acid-induced flowering in both L. paucicostata 151 and 381. |
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