Identification of GA3 in Neurospora crassa and Its Changes during Conidial Germination and Mycelial Growth |
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| Authors: | Yukiko Kawanabe Hisakazu Yamane Nobutaka Takahashi Teruko Nakamura |
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| Institution: | 1. Physiological Laboratory, Japan Women’s University, Bunkyo-ku, Tokyo 112, Japan;2. Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | GA3 was identified as a major GA in Neurospora crassa by gas chromatography/selected ion monitoring (GC/SIM) and its content was measured at various stages (0~96 hr after inoculation) of conidial germination and mycelial growth. The GA3 content in the fungus was 190ng/g dry weight at the initial stage and then decreased rapidly; that per liter culture decreased soon after the inoculation, and then increased to 17.6 ng 96 hr after inoculation. The GA3 concentration in the culture medium was around 10?11 m throughout the 96-hr incubation. The physiological role of endogenous GA in Neurospora crassa is also discussed. |
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