Protein Excretion through Bleb Formation by PE4LA,a Mutant of Escherichia coli |
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Authors: | Munekazu Tagawa Shigezo Udaka |
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Institution: | Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan |
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Abstract: | A mutant of E. coli (PE4LA) excreted approximately 15% of total cellular protein without cell lysis. The materials in the culture supernatant of the mutant were precipitated with 5% cold TCA. Protein, lipopolysaccharide (LPS), and phospholipid were found in a ratio of approximately 5:6:1. In electrophoretical analyses, exoproteins appeared to contain both periplasmic and outer membrane proteins.An electron microscopic study showed that PE4LA cells had many blebs around the cell surface and that these blebs were surrounded by double track layers. Some vesicles were also observed as free forms of blebs, while the parent cells had neither blebs nor vesicles. The vesicles appeared to be rich in LPS and lacked phosphatidylglycerol, compared to the outer membrane.The physiological and morphological data suggested alterations in the PE4LA cell surface, but what was altered remains obscure. It was concluded that PE4LA cells do not have a substantial increase in permeability, but rather have some defect in the cell envelope organization, which causes the formation of blebs with periplasmic proteins. |
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