A Phytotoxin,Betaenone C,and Its Related Metabolites of Phoma betae Fr |
| |
Authors: | Akitami Ichihara Hideaki Oikawa Masaaki Hashimoto Sadao Sakamura Tokuko Haraguchi Hiroshi Nagano |
| |
Affiliation: | 1. Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan;2. Department of Physiological Chemistry and Nutrition, Faculty of Medicine, The University of Tokyo, Tokyo 113, Japan |
| |
Abstract: | For rough quantitative analysis of genetically modified maize contents, rapid methods for measurement of the copy numbers of the cauliflower mosaic virus 35S promoter region (P35S) and MON810 construct-specific gene (MON810) using a combination of a capillary-type real-time PCR system with a plasmid DNA were established. To reduce the characteristic differences between the plasmid DNA and genomic DNA, we showed that pretreatment of the extracted genomic DNA by a combination of sonication and restriction endonuclease digestion before measurement is effective. The accuracy and reproducibility of this method for MON810 content (%) at a level of 5.0% MON810 mixed samples were within a range from 4.26 to 5.11% in the P35S copy number quantification. These methods should prove to be a useful tool to roughly quantify GM maize content. |
| |
Keywords: | genetically modified maize capillary-type real-time PCR system |
|
|