Ag + -Sensitive NADH Dehydrogenas in the Na+-Motive Respiratory Chain of the Marine Bacterium Vibrio alginolyticus |
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| Authors: | Makoto Asano Maki Hayashi Tsutomu Unemoto Hajime Tokuda |
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| Affiliation: | 1. Department of Membrane Biochemistry, Research Institute for Chemobiodynamics, Chiba University, 1-8-1 Inohana, Chiba, 280, Japan;2. Department of Enzymology, Research Institute for Chemobiodynamics, Chiba University, 1-8-1 Inohana, Chiba, 280, Japan |
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| Abstract: | Components of the Na+ -motive NADH : quinone oxi-doreductase segment in the respiratory chain of Vibrio alginolyticus were examined in membranes prepared from wild type, Na+ -pump-defective mutants, and a spontaneous revertant. Ag+ distinguished two kinds of respiratory NADH dehydrogenases. The Na+ -pump-defective mutants lacked Ag+ -sensitive NADH dehydrogenase activity. Incubation of the Ag+ -sensitive NADH dehydrogenase with solubilized membrane proteins of the mutant led to the reconstitution of Na+ -motive NADH : quinone oxidoreductase activity. We think that Ag+ -sensitive NADH dehydrogenase is an essential component of the respiratory Na+ pump of this organism. |
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