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Expression of Mature Human Interleukin 2 and Its Derivatives in Escherichia coli and Comparison of Their Biological Activity in Vitro |
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| Authors: | Hiromasa Miyaji Tatsunari Nishi Akiko Saito Shuichiro Maeda Kazunori Shimada Toshio Hirano |
| Affiliation: | 1. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, Machidashi Tokyo 194, Japan;2. Department of Biochemistry, Kumamoto University School of Medicine, 2–2–1 Honjo, Kumamoto 860, Japan;3. Department of Biochemistry, Institute for Medical Immunology, Kumamoto University School of Medicine, 2–2–1 Honjo, Kumamoto 860, Japan |
| Abstract: | A mature human interleukin 2 (hIl-2) and its derivatives that lacked the N-terminal portion were expressed in Escherichia coli under the control of the phage λ PL promoter. They accumulated in the form of insoluble inclusion bodies and accounted for about 30% of the total cellular protein. The mature hIl-2 and its derivatives were further purified and their in vitro biological activity was compared in an Il-2 microassay. The results suggested that the hIl-2 derivatives without the N- terminal three or five amino acids were as active as intact hIl-2 and that those without the N- terminal eight or nine amino acids were less active than the intact form. |
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