The Fermentative Formation of CDP-Choline by Haploid Cells of Yeasts and the Change of the Temperature-sensitivity of a Mutant of a Yeast,Saccharomyces rouxii |
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Authors: | Akira Kimura Minoru Hashimoto Haruhiko Mori |
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Affiliation: | 1. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan;2. Noda Institute for Scientific Research, Noda-Shi, Chiba, Japan |
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Abstract: | Phosphorylation of CMP and formation of CDP-choline were tested with various haploid cells of yeasts. Most of them had more or less the ability, but a mutant (Lys–M7, alpha type) of Saccharomyces rouxii was found to lack the ability. Further study revealed the change of the temperature-sensitivity of the mutant, which could not produce CDP-choline when treated at 37°C, whereas it could at 16°C. The growth of the mutant was more sensitive to temperatures than that of the wild strain. The former did not grow at 36.3°C, while the latter grew. |
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Keywords: | active site β- smallcaps smallerCapital" >d-glucosidase β- smallcaps smallerCapital" >d-fucosidase Dalbergia cochinchinensis |
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