首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Determination of CGTase from Bacillus ohbensis and Its Optimum pH Using HPLC
Authors:Michikatsu Sato  Yoshiaki Yagi  Hiroto Nagano  Tomoyuki Ishikura
Institution:Central Research Laboratories, Sanraku-Ocean Co, Ltd, 9-1, Johnan 4-chome, Fujisawa 251, Japan
Abstract:Glucose is widely known to be required during superoxide  /></span> generation in phagocytic cells. However, when an <span class= /></span> specific chemiluminescence probe with the <i>Cypridina</i> luciferin analog 2-methyl-6-(<i>p</i>-methoxyphenyl)-3, 7 -dihydroimidazo 1,2-a]pyrazin-3-one (MCLA) was used, about 60% of the chemiluminescence remained in stimulated macrophages in the presence of the glycolytic inhibitor 2-deoxyglucose. <span class= /></span> -nonspecific luminol-dependent chemiluminescence disappeared when the same drug was added. These results clearly demonstrate that the generation of <span class= /></span> by macrophages is not completely glucose-dependent, and strongly suggest that macrophages have both glucoseindependent NADPH-supplying pathway(s) and glucose dependent pathway(s) which generate reactive oxygen species other than <span class= /></span>.</td>
	  </tr> 
	  <tr>
	   <td align=
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号