Determination of CGTase from Bacillus ohbensis and Its Optimum pH Using HPLC |
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Authors: | Michikatsu Sato Yoshiaki Yagi Hiroto Nagano Tomoyuki Ishikura |
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Institution: | Central Research Laboratories, Sanraku-Ocean Co, Ltd, 9-1, Johnan 4-chome, Fujisawa 251, Japan |
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Abstract: | Glucose is widely known to be required during superoxide ![/></span> generation in phagocytic cells. However, when an <span class=](/na101/home/literatum/publisher/tandf/journals/content/tbbb19/1989/tbbb19.v053.i04/00021369.1989.10869415/20140909/images/medium/tbbb19_a_10869415_ilg0001.gif) ![/></span> specific chemiluminescence probe with the <i>Cypridina</i> luciferin analog 2-methyl-6-(<i>p</i>-methoxyphenyl)-3, 7 -dihydroimidazo 1,2-a]pyrazin-3-one (MCLA) was used, about 60% of the chemiluminescence remained in stimulated macrophages in the presence of the glycolytic inhibitor 2-deoxyglucose. <span class=](/na101/home/literatum/publisher/tandf/journals/content/tbbb19/1989/tbbb19.v053.i04/00021369.1989.10869415/20140909/images/medium/tbbb19_a_10869415_ilg0002.gif) ![/></span> -nonspecific luminol-dependent chemiluminescence disappeared when the same drug was added. These results clearly demonstrate that the generation of <span class=](/na101/home/literatum/publisher/tandf/journals/content/tbbb19/1989/tbbb19.v053.i04/00021369.1989.10869415/20140909/images/medium/tbbb19_a_10869415_ilg0002.gif) ![/></span> by macrophages is not completely glucose-dependent, and strongly suggest that macrophages have both glucoseindependent NADPH-supplying pathway(s) and glucose dependent pathway(s) which generate reactive oxygen species other than <span class=](/na101/home/literatum/publisher/tandf/journals/content/tbbb19/1989/tbbb19.v053.i04/00021369.1989.10869415/20140909/images/medium/tbbb19_a_10869415_ilg0002.gif) ![/></span>.</td>
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