Peptidoglycan of Pseudomonas aeruginosa |
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| Authors: | Akira Yanai Keijiro Kato Teruhiko Beppu Kei Arima |
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| Affiliation: | 1. Laboratory of Microbiology and Fermentation, Department of Agricultural Chemistry, University of Tokyo, Tokyo, Japan;2. Department of Microbiology, Osaka University Dental School, Osaka, Japan |
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| Abstract: | A practical method for preparing peptidoglycan from Ps. aeruginosa and E. coli was devised. After bacterial cells were dissolved in boiling 4% SDS solution, peptidoglycan was collected and washed with water by centrifugation. Peptidoglycan was treated further with pronase and lyophilized. The final preparation of peptidoglycan from Ps. aeruginosa appeared as a filmy coagulation in electron micrograph and its amino acid composition was determined as follows: Glu/Ala/A2pm/Mur/GlcN (100/183/104/61/98). The lysozyme digest showed the same pattern as that of E. coli peptidoglycan. N-Terminal analysis suggested that about half of the peptide chains was interbridged by the peptide bond between Ala and A2pm. The probable ratio of muropeptides in the peptidoglycan was estimated. |
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