Effect of Materials Released from Myofibrils by CAF (Ca2+-activated Factor) on Z-Disk Reconstitution |
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Authors: | Atsushi Suzuki Makoto Saito Hiroshi Sato Yoshinobu Nonami |
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Affiliation: | 1. Department of Animal Science, Faculty of Agriculture, University of Niigata;2. Electron Microscopic Laboratory, University of Niigata, Niigata, Japan |
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Abstract: | To investigate the constituents of Z-disk, reconstitution of Z-disk by incubating some proteins released from myofibrils by CAF(Ca2+-activated factor) with Z-disk-extracted fiber bundles was carried out and examined with electron microscope. The materials released from myofibrils by CAF have been bound in Z-disk region, and Z-disk extracted from myofibrils with a low ionic strength solution has been reconstituted. On the other hand, Z-disk removed from myofibrils by CAF has not been reconstituted by the same way. |
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