Separation of the Two Constituent Polypeptide Chains of Ricin D |
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Authors: | Gunki Funatsu Masaru Funatsu |
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Institution: | Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan |
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Abstract: | Two constituent polypeptide chains were isolated from performic acid-oxidized ricin D by DEAE-cellulose column chromatography in phosphate buffer, pH 7.0, containing 8m urea or from reduced-carboxymethylated ricin D by gel filtration on Sephadex G-75 followed by DEAE- cellulose column chromatography in Tris-HCl buffer, pH 8.5. Amino acid analyses of the isolated two chains revealed that they were distinct molecules possessing similar molecular weights of near 30,000 and linked by one disulfide bond in ricin D. |
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Keywords: | Bifidobacterium longum Escherichia coli shuttle vector pBLESl00 transformation by electroporation spectinomycin adenyltransferase AAD(9) |
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