Immunological Properties and Constituent Amino Acids of Three Xylanases Produced Inductively from Streptomyces sp. |
| |
Authors: | Masaki Marui Kotoyoshi Nakanishi Tsuneo Yasui |
| |
Institution: | Institute of Applied Biochemistry, University of Tsukuba, Sakura-mura, Niihari-gun, Ibaraki 305, Japan |
| |
Abstract: | Three xylanases produced inductively by methyl β-xyloside from Streptomyces sp. No. 3137 were purified to homogeneity. Rabbit antisera against two xylanases, X-I and X-II-B, were prepared.In double diffusion experiments, antiserum to X-I (anti-I serum) and antiserum to X-II-B (anti-II-B serum) formed a precipitation band with X-I, and X-II-B, respectively. No immunoprecipitate, however, was observed when xylanase X-II-A or X-II-B was tested against anti-I serum, and when X-I was done against anti-II-B serum. Anti-II-B serum formed a line of identity between X-II-A and X-II-B. The data indicate that X-II-A and X-II-B are closely related in immunology, while X-I is distinct.The amino terminals were an alanine residue for X-I and threonine residues for X-II-A and X-II-B by the dansyl chloride method. The carboxyl terminals were an aspartic acid residue for X-I and an alanine residue for X-II-B by carboxypeptidase Y method.Amino acid analysis showed that X-II-A and X-II-B had a large amount of glutamic acid and alanine residues, and a small amount of tyrosine, threonine, and serine residues compared with X-I. |
| |
Keywords: | |
|
|