Cellulase Production in Semisolid Cultures of Trichoderma viride |
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| Authors: | Leonora C. Vilela Angelo R. Torillo Alice T. de Ocampo Ernesto J. del Rosario |
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| Affiliation: | 1. Biological Research Center, National Institute of Science and Technology, Manila, Philippines;2. Department of Chemistry, University of the Philippines at Los Ba?os College, Laguna, Philippines |
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| Abstract: | Cellulase was produced by Trichoderma viride in semisolid cultures of rice bran, rice straw and rice hulls. T. viride QM 9414 generally produced higher cellulolytic activity on CM-cellulose (Cx activity) using rice bran-rice hull mixture (2:1 w/w) as substrate compared to strains ITCC 1433 and D 4014. It showed higher Cx activity on rice bran-rice straw mixtures than on rice bran-rice hull mixtures. Maximal extraction of the enzyme from mold bran was obtained with 0.05 m sodium citrate buffer, pH 3.5. |
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| Keywords: | low-molecular-weight fucoidan extract apoptosis calcium mitochondria breast cancer cell |
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