The Polysaccharide Releasing Phenomenon and Cell Envelope of the Inositol-Exacting Yeast in Inositol-Deficiency |
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| Authors: | Michio Dohi Kei Arima |
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| Institution: | Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Tokyo, Japan |
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| Abstract: | Inositol-deficiency abnormalized the cell envelope, wall and membrane, and the polysaccharides were released out of the cells in a greater amount in inositol-deficiency than in inositol-sufficiency. This release was most notable in the absence of inositol under the tested conditions. The released amount decreased when the culture was provided with inositol or phospholipids, or deprived of all growth-promoting vitamins. Gel filtration of released polysaccharides showed that the lower molecular fraction increased in inositol-deficiency than in sufficiency. DEAE-Sephadex chromatography of released polysaccharides showed the presence of fractions specific for inositol-deficiency which contained mannan and phosphates. Inositol-deficiency also caused enlarging of the pore size of the cell-wall. The relationship between the release of polysaccharides and the syntheses of cell-wall polysaccharides was investigated and discussed. |
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| Keywords: | green tea Camellia sinensis geranyl 6-O-α-L-arabinopyranosyl-β-D-glucopyranoside geranyl β- vicianoside monoterpene alcohol glycoside |
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