Synthesis of N-(9-Acridinyl)maleimide,a Fluorometrical Reagent for Thiol Compounds |
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| Authors: | Yasunori Nara Katura Tuzimura |
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| Institution: | Department of Food Chemistry, Faculty of Agriculture,Tohoku University, Sendai |
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| Abstract: | The authors have reported1) a new maleimide type fluorescent thiol reagent, N-(9-acridinyl)- maleimide (NAM). In this paper the syntheses of NAM and its coupling products with thiol compounds are presented. NAM was synthesized from 9-aminoacridine and maleic anhydride through dehydratic cyclization in polyphosphoric acid. NAM showed no substantial fluorescence but its coupling products with thiol compounds exhibited strong blue fluorescence. Application of NAM for the fluorometrical analysis of cysteine and glutathione are suggested. |
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| Keywords: | Oryza sativa β-amylase recombinant protein heat stability |
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