Protective Effects of Ca2+ on Cell Membrane Damage by Polygodial in Saccharomyces cerevisiae |
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| Authors: | Yoshihisa Yano Makoto Taniguchi Toshio Tanaka Susumu Oi Isao Kubo |
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| Affiliation: | 1. Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan;2. Division of Entomology and Parasitology, College of Natural Resources, University of California, Berkeley, California 94720, U.S.A. |
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| Abstract: | We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide. |
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| Keywords: | sphingomyelin lysosomes Niemann-Pick disease sphingomyelinase lysosomal acid phosphatase |
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