Determination of Reductones by High-Pressure Liquid Chromatographic Method |
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Authors: | Hitoshi Obata Wataru Tsuchihashi Tai Tokuyama |
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Affiliation: | Department of Chemistry, Faculty of Engineering, Kansai University, Suita, Osaka 564, Japan |
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Abstract: | Asaccharobacter celatus AHU1763 is a Gram-positive, obligate anaerobic, non-spore forming, rod-shaped bacteria that was successfully isolated from rat cecal content. Daizein was converted to equol via dihydrodaidzein by this bacterium. A crude enzyme that converted daidzein to dihydrodaidzein was detected mainly in the culture supernatant. The ability of this enzyme dropped after the culture supernatant was exposed to a normal atmospheric environment for even 5 min. Furthermore, the enzyme responsible for changing dihydrodaidzein to equol was detected mainly in the cell debris, which required anaerobic conditions for its activity. |
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Keywords: | Asaccharobacter celatus AHU1763 daidzein dihydrodaidzein equol |
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