Partial Purification of Intra- and Extra-cellular Cellulases from Pyricularia oryzae: with Reference to Optimum pH at Alkaline Side |
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| Authors: | Tadashi Sudo Hideo Nagayama Kinjiro Tamari |
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| Affiliation: | 1. Department of Agriculture Chemistry, Faculty of Agriculture, Tohoku University, Sendai;2. The Research Institute for Tuberculosis, Leprosy and Cancer, Tohoku University, Sendai |
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| Abstract: | In order to elucidate the relation between the difference in cellulase activity among various strains of P. oryzae and the optimum pH at alkaline side, and also to know the relation between the intra- and extra-cellulases, the elution patterns of the enzymes from the Sephadex G–100 column were compared and the occurrence of the enzyme fractions showing the optimum pH at alkaline side was investigated.The elution patterns of the intracellular cellulases were shown to be relatively constant, but those of the extracellular enzymes did not. The peak e appeared comparatively constant, but the peak c was considered to undergo some change during the excretion into the medium.The optimum pH at alkaline side was shown to occur in the peak e among five peaks on Sephadex G–100 of the partially purified intra- and extra-cellular cellulases. The peak seems to be significant for P. oryzae. |
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| Keywords: | Tulipa gesneriana dark purple anther delphinidin 3-O-(6-O-(α-rhamnopyranosyl)-β-glucopyranoside) delphinidin 3-O-(6-O- (2-O-acetyl-α-rhamnopyranosyl)-β-glucopy-ranoside) delphinidin 3-O-(6-O-(3-O-acetyl-α-rhamnopyranosyl)-β-glucopyranoside) |
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