Purification and Immunological Properties of NAD+-linked Malate Dehydrogenase Isozymes from Euglena gracilis |
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Authors: | Kazutaka Miyatake Koji Washio Akiho Yokota Yoshihisa Nakano Shozaburo Kitaoka |
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Affiliation: | Department of Agricultural Chemistry, University of Osaka Prefecture, Sakai, Osaka 591, Japan |
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Abstract: | We investigated the hypolipidemic effects of young persimmon fruit (YP) on apolipoprotein E-deficient C57BL/6.KOR-ApoEshl mice. These mice exhibited higher plasma cholesterols, except for high-density lipoprotein (HDL), and lower plasma HDL cholesterol than C57BL/6.Cr mice that had the same genetic background as the C57BL/6.KOR-ApoEshl mice. Male C57BL/6.KOR-ApoEshl mice (n=5) were fed a diet supplemented with dry YP, Hachiya-kaki, at a concentration of 5% (w/w) for 10 weeks. YP treatment significantly lowered plasma chylomicron, very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) cholesterols, and triglyceride, and this response was accompanied by an elevation of fecal bile acid excretion. In the liver, sterol regulatory element binding protein-2 gene expression was significantly higher in mice fed YP, while the mRNA and protein levels of the LDL receptor did not change. These results indicate that acceleration of fecal bile acid excretion is a major mechanism of the hypolipidemic effect induced by YP in C57BL/6.KOR-ApoEshl mice. |
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Keywords: | young persimmon fruit C57BL/6.KOR-ApoEshl mouse fecal bile acid excretion |
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