Isolation of Tryptic Peptides from the lie Chain of Ricin D and the Sequences of Some Tryptic Peptides Including N- and C-Terminal Peptides |
| |
Authors: | Gunki Funatsu Shinji Yoshitake Masaru Funatsu |
| |
Institution: | Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan |
| |
Abstract: | Twenty tryptic peptides were isolated from the performic acid-oxidized He chain of ricin D by Dowex 1 × 2 column chromatography followed by paper chromatography. The amino acids contained in these peptides accounted for 218 out of 266 residues in the whole protein. The amino acid sequences of nine peptides were determined by manual liquid phase or automatic solid phase Edman degradation, and N- and C-terminal sequences of the He chain of ricin D were established to be NH2–Ile–Phe–Pro–Lys–Gln–Tyr–Pro–Ile–Ile– and Cys–Ala–Pro–Pro–Pro–Ser–Ser–Gln–Phe, respectively. |
| |
Keywords: | Magnaporthe grisea Pyricularia oryzae appressorium formation surface recognition plant pathogenicity |
|