Application of Affinity Chromatography to Lipoxygenase

A simple electronic device was constructed which, in combination with a conventional titrator, records close approximation of the buffer capacity curve (β-pH curve) for the solution of unknown composition. Since the recorded curves provide the overall picture of the distribution of weak Brönsted acids in the solution on the pK_a axis, this apparatus may be useful in various fields such as chemistry of food and agricultural products and clinical medicine, where the characterization of the complex mixtures of weak electrolytes — carboxylic acids, amono acids, proteins, amines, phenols and etc. — are important.