Determination of the Molecular Weights of Gluten Proteins from Ferguson Plots in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis |
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| Authors: | Zen-ichiro Hamauzu Yoshiko Mabuchi Keiko Matsutaka |
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| Affiliation: | Department of Food Science, Faculty of Home Economics, Mukogawa Women ’s University, Ikebiraki-cho, Nishinomiya, Hyogo 663, Japan |
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| Abstract: | A mutant of Schizosaccharomyces pombe deficient in both superoxide dismutase with copper and zinc as cofactors and glutathione was hypersensitive to menadione, which intracellularly generates superoxide radicals, and showed short chronological lifespan with more oxidation of proteins. Disruption of the sir2 gene in the double mutant enhanced the short chronological lifespan without more enhanced protein oxidation. |
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| Keywords: | chronological aging sir2 glutathione superoxide dismutase Schizosaccharomyces pombe |
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