A New Differentiation Inducer of Friend Leukemia Cells,Trichostatic Acid |
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| Authors: | Hajimu Morioka Masaru Ishihara Misako Takezawa Kazuo Hirayama Eiichiro Suzuki Yasuo Komoda |
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| Affiliation: | 1. Central Research Laboratories, Ajinomoto Co., Ltd., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210, Japan;2. Research Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda-ku, Tokyo 110, Japan |
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| Abstract: | ![]()
FL-657B, which induced differentiation of Friend leukemia cells, was isolated from the culture fluid of Streptomyces sioyaensis and identified with trichostatic acid, a hydrolysis product of trichostatin A and C. FL-657B induced hemoglobin biosynthesis of both dimethyl sulfoxide-sensitive and -resistant Friend leukemia cells. FL-657B caused approximately 90% of the cells to be benzidine positive and reduced the growth to approximately 30~70% of the control at 2.42 μ/ml. Hemoglobin newly biosynthesized by the induction of FL-657B showed a UV absorption pattern similar to that from the normal mouse. |
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| Keywords: | xylose reductase site-directed mutagenesis coenzyme specificity Saccharomyces cerevisiae xylose fermentation |
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