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Preparation of Mutants Resistant to Catabolite Repression of Trichoderma reesei
Authors:Mikio Kawamori  Yasushi Morikawa  Yoriko Shinsha  Kenichiro Takayama  Seigo Takasawa
Institution:1. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Asahimachi, Machida-shi, Tokyo 194, Japan;2. Research and Development Division, Kyowa Hakko Kogyo Co., Ltd., Otemachi, Chiyoda-ku, Tokyo 100, Japan
Abstract:The development of agar plate screening techniques has allowed the isolation of mutants of Trichoderma reesei capable of synthesizing cellulase under the conditions of a high concentration of glucose. Mutants resistant to catabolite repression by glycerol or glucose were isolated on Walseth’s cellulose (WC) agar plates containing 5% glycerol or 5% glucose, respectively. Mutants resistant to catabolite repression by glycerol were not derepressed enough for the production of cellulase on WC agar plates containing 5% glucose or in flask cultures with a mixture of 1% Avicel and 3% glucose. On the contrary, two mutant strains resistant to catabolite repression by glucose (KDD-10 and DGD-16) produced large clearing zones on WC agar plates containing 5% glucose. Both strains could begin to produce CMCase even in the presence of residual glucose and finally produced 1.5 times the CMCase activity, in flask cultures on 1% Avicel and 3% glucose, than that with 1% Avicel alone. These results suggest that KDD-10 and DGD-16 are comparatively derepressed by glucose for cellulase production.
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