Characterization of Chromatin Subfractions Enriched in RNA from Lactating Mammary Gland of the Rat |
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| Authors: | Masaaki Hirose Keiko Anabuki Masatoshi Maki Kiyozo Hasegawa Hideo Chiba |
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| Affiliation: | 1. Department of Food Science and Nutrition, Nara Women’s University, Nara, Nara 630, Japan;2. Department of Food Science and Technology, Kyoto University, Kyoto, Kyoto 606, Japan |
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| Abstract: | Mammary gland chromatin from lactating rats was fractionated according to the Mg2+-solubility method after mild digestion with DNase II. The chromatin properties were compared between the Mg2+-soluble (S2) and the Mg2+-insoluble (P2) fractions. The weight ratio of RNA to DNA was much greater in the S2 fraction than in the P2 fraction (S2, 0.92; P2, 0.03). The DNA repeat length of the nucleosome was very similar between the two fractions (S2, 193 ± 7; P2, 1% ± 8 nucleotide pairs). A significant difference was seen in electrophoretic pattern of H1 histone between the two fractions in the acid-urea gel system. Nonhistone proteins with molecular weights higher than about 40,000 dalton were found to be enriched in the S2 fraction. |
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