Preparation of Triacylglycerol Molecular Species by Interesterification Using Endocellular Lipase in n-Hexane |
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Authors: | Masami Kimura Kiyozo Hasegawa Hitoshi Takamura Teruyoshi Matoba |
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Institution: | 1. Division of Human Life and Environmental Sciences, Graduate School of Human Culture, Nara 630, Japan;2. Department of Food Science and Nutrition, Faculty of Home Economics, Nara Women’s University, Nara 630, Japan |
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Abstract: | The interesterification of triacylglycerol with fatty acid was done to prepare triacylglycerol molecular species. Optimum operating conditions for the interesterification using a 1,3-positional specific endocellular lipase from Rhizopus japonicus NR400 in a batch system were investigated. The reaction was done at 40°C for 5 hr in the following system: Trioleoylglycerol-palmitic acid = 1:3.5 (mol/mol), 10 ml n-hexane/g trioleoylglycerol, and 2500 units of enzyme/g trioleoylglycerol. Under these conditions, the content of palmitoyl groups in 1,3-positions of triacylglycerol was about 60 mol%. Additional interesterification (2-cycle reaction) using palmitic acid and the novel triacylglycerol prepared by one-step interesterification (1-cycle reaction) resulted in a preparation of highly pure 1,3-dipalmitoyl-2-oleoylglycerol. |
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Keywords: | carbohydrate-binding module xylanase thermostability differential scanning calorimetry (DSC) |
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