Two Forms of Glucoamylase from Mucor rouxianus |
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Authors: | Yoshiki Yamasaki Akira Tsuboi Yukio Suzuki |
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Institution: | Institute for Agricultural and Biological Sciences, Okayama University, Kurashiki, Okayama-ken |
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Abstract: | Both of the two forms of glucoamylase (glucoamylases I and II) from the wheat bran culture of Mucor rouxianus hydrolyzed amylopectin, amylose, glycogen, soluble starch, maltotriose, and maltose, but did not act on isomaltose and isomaltotriose. Phenyl α-maltoside was hydrolyzed into glucose and phenyl α-glucoside by both glucoamylases. Maltose was hydrolyzed about one-fifth as rapidly as amylopectin. Both enzymes produced glucose from amylopectin, amylose, glycogen, soluble starch in the yields of almost complete hydrolysis. They hydrolyzed amylose with the inversion of configuration, producing the β-anomer of glucose. Glucoamylase II hydrolyzed raw starch at 3-fold higher rate than glucoamylase I. The former hydrolyzed rice starch almost completely into glucose, whereas the latter hydrolyzed it incompletely (nearly 50%). |
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Keywords: | Ehrlich pathway isobutanol Saccharomyces cerevisiae valine biosynthetic pathway |
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