Generation of Superoxide and In Vitro Inactivation of Viruses by Aldopentoses |
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| Authors: | Naoki Kashimura Ikuo Sato Zenzaburo Kumazawa Hirokazu Kuno Shiro Koyama Masaru Kitagawa |
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| Institution: | Laboratory of Pesticide Chemistry, Department of Agricultural Chemistry, Mie University, Tsu, Mie 514, Japan |
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| Abstract: | Ren-1 renin is synthesized in the kidney of every mouse. Ren-2 renin has been observed in the submandibular gland (SMG) of male mice carrying two renin genes. However, it is not known if Ren-2 renin is in the kidney and blood of the two-renin gene mice. In this study, a direct ELISA for Ren-2 renin (SMG renin) was established by a sandwich method. This ELISA could measure the Ren-2 active renin in the range from 1 to 100ng and distinguish Ren-2 active renin from not only Ren-1 renin but also Ren-2 prorenin. By a combination of this assay system and conventional methods, the pro-form as well as the active form of Ren-2 renin was found in the kidney and plasma of male AKR mice carrying two-renin genes. |
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