Structural Analysis of an Extracellular Polysaccharide of Porodisculus pendulus |
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Authors: | Yoshiaki Iwamuro Michiko Murata Koichiro Kanamaru Yoichi Mikami Takuro Kisaki |
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Affiliation: | The Central Research Institute, Japan Tobacco and Salt Public Corporation, 6–2 Umegaoka, Midori-ku, Yokohama 227, Japan |
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Abstract: | The aroL gene, encoding shikimate kinase of Brevibacterium lactofermentum, a coryneform glutamic acid-producing bacterium, was cloned. Recombinant plasmids containing the aroL gene caused elevated levels of shikimate kinase synthesis in B. lactofermentum. It was found that in addition to the aroL gene, the aroB and aroE genes, encoding dehydroquinate synthase and shikimate dehydrogenase, respectively, also existed on these recombinant plasmids, in complementation tests with various Escherichia coli and B. lactofermentum aromatic amino acid auxotrophs. The aroL, aroB and aroE genes of B. lactofermentum are located closely on the cloned DNA fragment, in that order. It was shown that at least these three aro genes form a cluster on the chromosome of B. lactofermentum. |
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Keywords: | dechlorination 2,4-dichlorophenol degradation of 2,4-dichlorophenol Mortierella sp. |
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